What is Prolotherapy? Prolotherapy is a type of treatment used by stimulating the healing factors in the body to improve the skeletal and muscular system in case of pain or damage. How is Prolotherapy Done? A liquid prepared to stimulate reparative factors is injected into the aching or damaged area. The purpose of this fluid is to stimulate the repair cells in the painful or damaged area and to treat the discomfort in that area by the body itself. After prolotherapy applied under local anesthesia, the patient can be discharged on the same day.